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Restoring Hair and Scalp Health: Ceramide-2 Analog for Conditioning and Barrier Benefits

Contact Author Xianting Lin and Jiangzhong Yang, Beauty Hi-tech Innovation Co., Ltd., Kobe, Japan; Yihua Jiang and Jie Lu, Symrise, Shanghai
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Ceramides are found in skin basal epidermal cells and dermal fibroblasts,1 and play a crucial role in the barrier function of the epidermis. It is well known that when the amount of ceramides present in the stratum corneum decreases, the potential for trans-epidermal water loss (TEWL) increases, especially during cold months2 and in aged skin.3 Furthermore, ceramides play a major role in the antimicrobial defense of scalp skin, especially against dandruff.4

Additionally, along with 18 methyl eicosanoic acids and cholesterols, ceramides are one of three types of lipids found inside the cuticle of human hair,5 functioning as a glue to keep the scales in place. In damaged hair, however, ceramides are often significantly decreased or missing.

Since ceramides are essential for protecting both skin and hair, the natural conclusion would be to use them as cosmetic ingredients for the skin (including scalp) and hair, to recover lost functions in these substrates. However, naturally occurring ceramides are difficult to extract and hence, can carry a high cost. This limits their potential in cosmetic products.

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In response to these challenges, a structural analog to naturally occurring ceramide-2 (see Figure 1) was developeda for application in skin, scalp and hair care. The present study investigates its performance when formulated in hair care products.

Materials and Methods

Scalp skin barrier repair: To assess barrier repair, this study was conducted by treating the dry, winter volar forearm skin of 20 test subjects with 2% sodium dodecyl sulfate (SDS) for 24 hr to disrupt the skin barrier, followed by application of 2 mg/cm2 of the test formulas twice daily for five days. The base test formulas, with or without 0.5% ceramide-2 analog, ceramide-2 or ceramide-3, included: water 71.05%, caprylic/capric triglyceride 20%, glyceryl stearate 2.0%, cetearyl alcohol 2.0%, glyceryl stearate citrate 1.0%, dimethicone 0.5%, sodium hydroxide 0.5% and preservatives. Reparative effects were determined by TEWL measurements.

Hair conditioning via dynamic combing: To evaluate the conditioning performance of the ceramide-2 analog, dynamic combing tests were performedb on healthy Asian hair bundlesc using a test shampoo containing 1% of the analog. This test measured the required combing force through the hair bundle at five different evaluation phases: lathering, during rinsing first half, during rinsing second half, after rinsing and air-dried. Before treatment, initial wet combing forces were also measured in the freshly washed hair bundle. Thus, the larger the number, the more difficult the combing and the less conditioned the hair.

The test shampoo included: 12% sodium laureth sulfate, 3% cocamidopropyl betaine, 1.5% glycol distearate, 1% ceramide-2 analog, 0.3% polyquaternium-10, 0.35% fragrance, 0.2% NaCl, preservatives and citric acid to pH 6.

Hair repair via microscopic imaging: To observe potential hair repair effects, a test conditioner with or without 1% ceramide-2 analog was applied to Asian hair samples, which were examined under an electron microscoped. The hair conditioner formula used for this study included: 2.74% behentrimonium chloride, 2.53% cetyl alcohol, 2.31% stearyl alcohol, 1% ceramide-2 analog, 0.35% fragrance and preservatives.

The hair preparation procedure included: 1) applying 1 g conditioner, as above, onto the hair bundle; 2) rinsing hair for 15 sec with 38˚C water; 3) combing hair 30x without rinsing; 4) combing hair 10x with rinsing; and 5) drying naturally overnight.

Contact angle and hair hydrophobicity: As an indicator of hair health, contact angle measurements between water and the damaged Asian hair were made using the same electron microscoped. The larger the contact angle, the higher the hair surface hydrophobicity—meaning the healthier the hair. The hair preparation procedure followed that described above for hair repair measurements.

Hair tensile testing: Also indicating hair’s health and strength, tensile measurements were taken of the maximum load 10 damaged Asian hair fibers could withstand before reaching the breaking pointe. Again, the hair preparation procedure and test conditioner used were identical to those described above for hair repair assessments. Test conditions were: 20 ± 5°C, at 65% ± 10% RH at 60 mm/min.

Results indicated the ceramide-2 analog acted as an effective replacement for natural ceramides in hair.

Hair Assessment Results

TEWL: As shown in Figure 2, a 0.5% ceramide-2 analog emulsion significantly improved the skin barrier, as measured by TEWL, in comparison with untreated and placebo-treated skin. These results were similar to those delivered by natural ceramide-2 and ceramide-3. The ceramide-2 analog formula showed 82% recovery, compared with untreated and undamaged skin; ceramides 2 and 3 showed 86% and 90% recovery, respectively.

Dynamic hair combing: As shown in Figure 3, the shampoo with 1% ceramide-2 analog showed significantly reduced combing forces during the rinsing second half and dried stages, compared with the control shampoo. Although the other stages did not indicate statistically significant differences, improvements were noted (data not shown). These results confirm the ceramide-2 analog improved hair combability.

Microscopic hair imaging: As can be seen in Figure 4, exemplified by one sample strand, the hair surface treated with the ceramide-2 analog conditioner was apparently smoother and showed less cuticle lift than hair conditioned without it.

Contact angle measurements: As shown in Figure 5, hair treated with the ceramide-2 analog-containing conditioner showed higher surface hydrophobicity.

Tensile testing: Figure 6 shows the average result of the 10 tested fibers. Here, one can see the hair treated with the ceramide-2 analog conditioner broke after a larger maximum load value than the control hair. This suggested the analog strengthened the hair fibers.

Discussion and Conclusions

As stated, naturally-occurring ceramides are known for their functions in protecting the skin, scalp and hair. However, cost has been a key limiting factor to their use. As such, an analog to ceramide-2 was developed as an alternative and was evaluated here for its potential in hair care.

The experimental results indicate the ceramide-2 analog acts as an effective replacement for natural ceramides in terms of hair conditioning benefits. Specifically, it helped to improve the combability of hair, flattened uplifted cuticles, restored the hydrophobicity of damaged hair and increased the strength of damaged hair. The ability of the analog to reduce TEWL in skin suggests scalp care applications as well.

It is also important to note the ceramide-2 analog did not negatively affect the fundamental properties of the shampoo and conditioner test formulas, such as appearance and viscosity, at the tested incorporation levels (data not shown).

It is hypothesized that the performance of the ceramide-2 analog is primarily due to its structural similarity and hydrophilicity-hydrophobicity balance to its natural analog, which is achieved with its hydrophilic head comprising an amide group, an ester group and a hydroxyl group, in addition to two C16 hydrophobic tails.

In conclusion, the ceramide-2 analog effectively improved parameters indicative of repairing damaged scalp and hair when applied in test shampoo and conditioner formulas. It is therefore recommended as an ingredient for hair repair and scalp care applications.

References

  1. G Vielhaber et al, Localization of ceramide and glucosylceramide in human epidermis by immunogold electron microscopy, J Invest Dermatol 117 1126–36 (2001)
  2. J Rogers et al, Stratum corneum lipids: The effect of aging and the seasons, Arch Dermatol Res 288 765–70 (1996)
  3. AV Rawlings et al, Changes in lipids in the skin aging process, IFSCC vol I 31–45 (1993)
  4. CR Harding et al, Dandruff: A condition characterized by decreased levels of intercellular lipids in scalp stratum corneum and impaired barrier function, Arch Dermatol Res 294 221-30 (2002)
  5. JR Smith et al, Lamellar subcomponents of the cuticular cell membrane complex of mammalian keratin fibers show friction and hardness contrast by AFM, J Microsc 206(3) 182–193 (2002)

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Figure 1. Structure of ceramide-2 analog (a) and natural ceramide-2 (b)

Figure 1. Structure of ceramide-2 analog (a) and natural ceramide-2 (b)

A structural analog (C16/C18) (b) to naturally occurring ceramide-2 (C16/C16) (a) was developed for application in skin, scalp and hair care.

Figure 2. Effects of natural ceramides and ceramide analog on TEWL measurements

Figure 2. Effects of natural ceramides and ceramide analog on TEWL measurements

A 0.5% ceramide-2 analog emulsion significantly improved the skin barrier, as measured by TEWL, in comparison with untreated and placebo-treated skin.

Figure 3. Dynamic combing test of hair treated with shampoos

Figure 3. Dynamic combing test of hair treated with shampoos

The shampoo with 1% ceramide-2 analog showed significantly reduced combing forces during the rinsing second half and dried stages, compared with the control shampoo.

Figure 4. Microscopic imaging of hair treated with conditioners (500×)

Figure 4. Microscopic imaging of hair treated with conditioners (500×)

The hair surface treated with the ceramide-2 analog conditioner was apparently smoother and showed less cuticle lift than hair conditioned without it.

Figure 5. Contact angle measurements for hair treated using conditioners without (a) and with the ceramide-2 analog (b)

Figure 5. Contact angle measurements for hair treated using conditioners without (a) and with the ceramide-2 analog (b)

Hair treated with the ceramide-2 analog-containing conditioner showed higher surface hydrophobicity.

Figure 6. Tensile test for hair treated with conditioners

Figure 6. Tensile test for hair treated with conditioners

Here, one can see the hair treated with the ceramide-2 analog conditioner broke after a larger maximum load value than the control hair.

Footnotes (CT1802 Yang)

aCeramideBio (INCI: Cetylhydroxyproline Palmitamide), Symrise Co., Ltd.

b SK-3A, Techno Hashimoto Co., Ltd.

cBeaulax Co., Ltd.

dKeyence Co., Ltd.

eAGS-X Series electronic universal testing machine, Shimadzu Corp.

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