Mechanisms of Tape Stripping and Protein Quantification*

Mar 1, 2010 | Contact Author | By: Ali Alikhan, MD, and Howard I. Maibach, MD
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Title: Mechanisms of Tape Stripping and Protein Quantification*
tape-strippingx gravimetricx colorimetricx spectroscopicx biological responsex TEWLx epidermisx
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Keywords: tape-stripping | gravimetric | colorimetric | spectroscopic | biological response | TEWL | epidermis

Abstract: In this article, several methods to quantify the protein collected by tape stripping are described, including traditional gravimetric methods as well as novel colorimetric and visible spectroscopic techniques. Further, one colorimetric method is described to effectively determine the keratolytic efficacy of various materials in vivo, suggesting additional roles for this method.

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A Alikhan and HI Maibach, A dermatological view—Mechanisms of tape stripping and protein quantification, Cosm & Toil 125(3) 40-49 (Mar 2010)

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Stratum corneum (SC) adhesive tape stripping has been utilized in the measurement of SC mass, barrier function, drug reservoir and percutaneous penetration of topical substances for nearly 50 years. The process involves a methodical, relatively noninvasive layer-by-layer removal of the outermost epidermal cell layers. Complete SC removal may require more than 70 tape strips.

The quantity of SC harvested diminishes with each sequential strip, possibly due to increased SC cohesiveness in deeper layers. Thus, the mass of any single strip depends upon the mass removed by the prior strip. SC removal may rely on the interaction between the adhesive stripping force and the cohesive intercellular force.

In this article, several methods to quantify the protein collected by tape stripping are described, including traditional gravimetric methods as well as novel colorimetric and visible spectroscopic techniques. Further, one colorimetric method is described to effectively determine the keratolytic efficacy of various materials in vivo, suggesting additional roles for this method.

Excerpt Only This is a shortened version or summary of the article you requested. To view the complete article, please log in or create an account. Registration is Free!

 

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Table 1. Three studies quantifying SC thickness in vivo

Table 1 summarizes the results from the Kalia, Schwindt and Pirot studies quantifying SC thickness.

Table 2. Relationship between protein removal and TEWL3

Furthermore, 5-7 um of SC removal resulted in significant TEWL elevations, a depth unobtainable by the rayon tape (see Table 2).

Table 3. Studies using colormetric protein assay to measure keratolytic potential

The results obtained from three keratolytics from colorimetric protein assays, as described by Dreher et al.

Footnotes

a The Bio-Rad Detergent Compatible Protein Assay Kit used for this study is manufactured by Bio-Rad Laboratories, Hercules, Calif., USA.
b The Hitachi U-2001 UV-vis Spectrophotometer used for this study is manufactured by Hitachi, Tokyo.

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