Tape Stripping Method in Humans: Comparison of Evaporimetric Methods

Feb 1, 2009 | Contact Author | By: Hongbo Zhai, MD, and Howard I. Maibach, MD, University of California School of Medicine
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Title: Tape Stripping Method in Humans: Comparison of Evaporimetric Methods
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The stratum corneum (SC) has been well recognized as a principal water barrier of the skin. It is a cellular tissue, a fabric of cornified cells creating a tough, flexible, coherent membrane, acting as a two-way barrier, minimizing water loss, electrolytes and other body constituents, and decreasing the entry of noxious substances from the external environment.1,2 Maintenance of the SC structural integrity is critical to barrier function. Transepidermal water loss (TEWL) documents the integrity of SC water barrier function, and is a sensitive indicator of skin water barrier alteration.3 Healthy SC typically has a water content of 10–20%;2 the TEWL can be dramatically altered if this barrier function is perturbed by physical, chemical, therapeutic or pathological factors.4-7

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Table 1. Mean, standard deviation (S.D.) and (inter-subject) coefficient of variation (CV%) of TEWL on stripped skin

  After 10 strips   After 20 strips     
  TEWL CV TEWL CV
  (g/m2/hr) (%) (g/m2/hr)  (%)
Open chamber device   2.2 ± 0.9  41.7 3.9 ± 1.3 33.5
 Closed chamber device 2.4 ± 1.4  60.5 4.6 ± 2.2 47.8

Figure 1. The scatter of TEWL values with two devices after 10 (s) and 20 (n) strippings. Data were subtracted from baseline and shown as mean values.

Figure 1. The scatter of TEWL values with two devices after 10 (s) and 20 (n) strippings. Data were subtracted from baseline and shown as mean values.
Figure 1. The scatter of TEWL values with two devices after 10 (s) and 20 (n) strippings. Data were subtracted from baseline and shown as mean values.

Footnotes

a D-Squame tape discs, CuDerm Corp., Dallas, Tex. D-Squame is a registered trademark.
b Tewameter, TM 210, Courage & Khazaka, Cologne, Germany, and Acaderm Inc., Menlo Park, Calif., USA. Tewameter is a registered trademark.
c Vapometer SWL-3, Delfin Technologies Ltd, Kuopio, Finland. Vapometer is a registered trademark.
d SigmaStat, a product of SPSS Science, Chicago, Ill., USA. SigmaStat is a registered trademark.

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