In vitro

Collagens I and III, and Elastin Activation for Anti-aging

Feb 24, 2014D. Auriol and G. Redziniak Libragen; and H. Chajra, K. Schweikert and F. Lefevre, Induchem

As an alternative to semi-invasive facial rejuvenation techniques, the authors developed an active ingredient to reactivate senescent fibroblasts by stimulating metabolic pathways for collagens I and III, and elastin. The biological activity of the resulting ingredient is investigated here using in vitro models, ex vivo explants and human volunteers.

Measuring the Water Content of Hair

Feb 21, 2014Trefor A Evans, T.A Evans LLC

Consumers have demonstrated a clear desire for hair that isn’t “dried out” while also demonstrating a clear distaste for the effects of high humidity on hair. To find the balance in creating products, it is necessary to have an accurate means of measuring hair’s water content. This article describes equipment used to perform this task while highlighting experimental variables that can produce suspect results and lead to incorrect conclusions.

Analyzing Deposition from Rinse-off Hair Products

Jan 13, 2014Qing Huang, Zhen-Wu Mei, Koji Takata and Jianzhong Yang, Beauty & Health Innovation Co., Ltd.

The most common approach to determine ingredient deposition on hair is to analyze the treated tresses, but this poses several challenges. Instead, the authors describe a novel approach based on determining the amount of ingredient collected in the rinse water, and back-calculating the amount deposited on hair. Development and validation efforts discussed here use polydimethylsiloxane as a model compound.

Mature and Immature Corneocyte Detection Force Distance Curves vs. Microfluorometry

May 1, 2013Anthony J. Ribaudo

Here, the author compares two methods to determine the maturity of corneocytes based on their cross-linking that could be used to evaluate the anti-aging effects of molecular agents. The first utilizes microfluorometry, while the second involves F-D curves generated via contact mode AFM. Both methods successfully detected differences in mature or immature corneocytes with 95% confidence.