A Rapid and Sensitive In vitro Method to Ascertain Antioxidative Capacity*

By: Hongbo Zhai, MD, and Howard I. Maibach, MD, University of California San Francisco

Posted: January 29, 2010, from the February 2010 issue of Cosmetics & Toiletries.

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Additionally, NAOC retained a wide solubility range, from a hydrophilic solvent to the five liphophilic solvents tested, whereas the solubility of idebenone was relatively narrow. This compatibility would give NAOC an added advantage when considering its combination with other cosmetic ingredients.

Conclusion
Providing a reliable analytical method to detect the antioxidative capacities of even low antioxidant concentrations in a sample is of major interest, and photochemiluminescence analysis has advantages over other methodologies.^4-11 Taken together, it is a simple, sensitive, economical, convenient, and reliable method to provide a practical preclinical screening of antioxidant materials—prior to animal/human trials.

References
Send e-mail to zhaiH@derm.ucsf.edu.
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*Revised with the permission of Blackwell Publishing from: H Zhai, M Cordoba-Diaz, C Wa, X Hui and HI Maibach, Determination of the antioxidative capacity of an antioxidant complex and idebenone: An in vitro rapid and sensitive method, Journal of Cosmetic Derm 7(2) 96-100 (2008).