Build a solid foundation in science, formulation and product development—find out more!
Most Popular in:
A Rapid and Sensitive In vitro Method to Ascertain Antioxidative Capacity*
By: Hongbo Zhai, MD, and Howard I. Maibach, MD, University of California San Francisco
Posted: January 29, 2010, from the February 2010 issue of Cosmetics & Toiletries.
page 4 of 4
Additionally, NAOC retained a wide solubility range, from a hydrophilic solvent to the five liphophilic solvents tested, whereas the solubility of idebenone was relatively narrow. This compatibility would give NAOC an added advantage when considering its combination with other cosmetic ingredients.
Providing a reliable analytical method to detect the antioxidative capacities of even low antioxidant concentrations in a sample is of major interest, and photochemiluminescence analysis has advantages over other methodologies.4-11 Taken together, it is a simple, sensitive, economical, convenient, and reliable method to provide a practical preclinical screening of antioxidant materials—prior to animal/human trials.
Send e-mail to zhaiH@derm.ucsf.edu.
1. KJ Davies, An overview of oxidative stress, IUBMB life 50 241-244 (2000)
2. JJ Thiele, F Dreher and L Packer, Antioxidant defense systems in skin, in Cosmeceuticals, Drugs vs. Cosmetics, P Elsner and HI Maibach, eds, New York: Marcel Dekker (2000) pp 145-187
3. JJ Thiele, Oxidative targets in the stratum corneum. A new basis for antioxidative strategies, Skin Pharmacol Appl Skin Physiol 14 (suppl 1) 87-91 (2001)
4. F Dreher and HI Maibach, Protective effects of topical antioxidants in humans, in Oxidants and Antioxidants in Cutaneous Biology, JJ Thiele and P Elsner, eds, Basel: Karger (2001) pp 157-164
5. F Stäb, R Wolber, T Blatt, R Keyhani and G Sauermann, Topically applied antioxidants in skin protection, Methods Enzymol 319 465-478 (2000)
6. R Kohen, Skin antioxidants: Their role in aging and in oxidative stress—New approaches for their evaluation, Biomed Pharmacother 53 181-192 (1999)
7. F Dreher, B Gabard, DA Schwindt and HI Maibach, Topical melatonin in combination with vitamins E and C protects skin from ultraviolet-induced erythema: A human study in vivo, Br J Dermatol 139 332-339 (1998)
8. H Zhai, S Behnam, CD Villarama, M Arens-Corell, MJ Choi and HI Maibach, Evaluation of the antioxidant capacity and preventive effects of a topical emulsion and its vehicle control on the skin response to UV exposure, Skin Pharmacol Appl Skin Physiol 18 288-293 (2005)
9. H Zhai, MJ Choi, M Arens-Corell, BA Neudecker and HI Maibach, A rapid, accurate and facile method to quantify the antioxidative capacity of topical formulations, Skin Res Technol 9 254-256 (2003)
10. DH McDaniel, BA Neudecker, JC Dinardo, JA Lewis and HI Maibach, Idebenone: A new antioxidant: Part I. Relative assessment of oxidative stress protection capacity compared to commonly known antioxidants, J Cosmet Dermatol 4 10-17 (2005)
11. DH McDaniel, BA Neudecker, JC Dinardo, JA Lewis and HI Maibach, Clinical efficacy assessment in photodamaged skin of 0.5% and 1.0% idebenone, J Cosmet Dermatol 4 167-173 (2005)
12. L Packer and G Valacchi, Antioxidants and the response of skin to oxidative stress: Vitamin E as a key indicator, Skin Pharmacol Appl Skin Physiol 15 282-290 (2002)
13. IN Popov and G Lewin, Photochemiluminescent detection of antiradical activity. IV: Testing of lipid-soluble antioxidants, J Biochem Biophys Methods 11 1-8 (1996)
14. I Popov and G Lewin, Antioxidative homeostasis: Characterization by means of chemiluminescent technique, Methods Enzymol 300 437-456 (1999)
*Revised with the permission of Blackwell Publishing from: H Zhai, M Cordoba-Diaz, C Wa, X Hui and HI Maibach, Determination of the antioxidative capacity of an antioxidant complex and idebenone: An in vitro rapid and sensitive method, Journal of Cosmetic Derm 7(2) 96-100 (2008).