Comparing Methods to Measure Porcine Skin Integrity In Vitro

May 6, 2003 | Contact Author | By: S. Richert, A. Schrader and K. Schrader, Institut Dr. Schrader Creachem GmbH
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Keywords: in vitro testing | skin penetration | skin integrity | porcine skin model

Abstract: To confirm the integrity of porcine ski prior to penetration tests, published studies show that measuring transepidermal water loss, transdermal electrical resistance or caffeine penetration levels are often chosen techniques. New results demonstrate that

Initial evaluation of the in vitro porcine skin model has demonstrated its feasibility and consequently led to the OECD guideline TG428. Fresh porcine skin, which has been reduced to horny layer and epidermis plus some dermal tissue, is often used to determine the percutanous absorption of test substances in different vehicles. Punched circular pieces of porcine skin are placed in suitable diffusion chambers for testing.