In vitro Buffering Capacity of Human Skin Layers*

Jun 1, 2012 | Contact Author | By: Y. Zheng, MD, PhD, Third Affiliated Hospital of Sun Yat-sen University, and H.I. Maibach, MD, University of California at San Francisco
Your message has been sent.
(click to close)
Contact the Author
Save
This item has been saved to your library.
View My Library
(click to close)
Save to My Library
Title: In vitro Buffering Capacity of Human Skin Layers*
bufferingx stratum corneumx irritantsx in vitrox
  • Article
  • Media
  • Keywords/Abstract
  • Related Material

Keywords: buffering | stratum corneum | irritants | in vitro

Abstract: Previous studies demonstrate that skin buffering capacity can be measured in vitro by applying several concentrations of hydrogen chloride (HCl) and sodium hydroxide (NaOH) on skin and evaluating the pH change pre- and post-dosing. Here, the authors employed this technique to evaluate the buffering capacity of skin layers including intact SC, denuded SC and dermis skin samples.

View citation for this article

Y Zheng and HI Maibach, A dermatological view—In vitro buffering capacity of human skin layers, Cosm & Toil 127(6) 426-429 (Jun 2012)

Market Data

  • Peptides are spreading beyond their core facial care market; melanin-activating peptides (MAPs) are gaining momentum in self-tanning.
  • Self-tanning accounts for only 5% of the global sun care market.
  • Self-tanning is restricted to developed markets, with North America and Western Europe making up almost 80% of total sales.
view full article

Related Material

Market Intelligence

* Modified with permission from Y Zheng, Buffering capacity of human skin layers: In vitro, Skin Research and Technology (2011).11

Normal stratum corneum (SC) is acidic, with typical pH ranges from 4 to 6, and while skin exposed to aqueous acid or alkaline solutions exhibits changes in pH, it may rapidly restore to the baseline values. This phenomena is called buffering capacity. Many factors contribute to skin’s buffering capacity including keratin, proteins, sweat, SC thickness, free amino acids and other water-soluble epidermis constituents.1 Previous studies demonstrate that skin buffering capacity can be measured in vitro by applying several concentrations of hydrogen chloride (HCl) and sodium hydroxide (NaOH) on skin and evaluating the pH change pre- and post-dosing.2 Here, the authors employed this technique to evaluate the buffering capacity of skin layers including intact SC, denuded SC and dermis skin samples.

Materials

Skin: Human skin was dermatomed to 500 μm thickness and frozen at -20°C. Defrosted skin samples were removed by 40 continuous tape strips. The epidermis samples were heat-separated for 30 sec in a 60°C water bath.3 Skin samples were acclimatized for at least 30 min prior to baseline measurements.

Irritants (model acid and base): NaOH, HCl solutions and water (HPLC grade) were prepared as previously described.2

pH measurement: A skin pH metera was used to perform pH measurements. The electrode was mounted directly on the skin surface to obtain recordings; the principle of this device and measuring method were previously published.4, 5

TEWL measurement: An evaporation meterb was utilized to measure TEWL as previous described.6, 7 TEWL readings are expressed in g/m2 per hr.

Methods

Split skin samples, approximately 5 cm2 in surface area, dermatomed to 500 μm thickness, were mounted on liquid scintillation vials filled with saline, 0.9% sodium chloride (NaCl), as receptor fluid. After initial preparations, samples were equilibrated for 30 min. Prior to dosing, the baseline pH and TEWL values were recorded. Each sample was dosed with 3.18 μL/cm2 of corresponding acid and base. Skin washed with de-ionized water and untreated skin were used as blank and negative controls, respectively. Immediately after dosing, as well as at 10 min and 30 min intervals, pH measurements were performed. Thirty minutes later, skin surfaces were washed twice with 1 mL of de-ionized water for 10 sec. The pH levels of washed skin were taken immediately and at 10 min and 30 min intervals. At the end of measurements, skin was cut with a punch and weighed by equi-armbalance.

Results

pH changes: pH values significantly changed (p < 0.05) immediately following NaOH exposure. Higher concentrations induced the greatest pH changes. Within a short time after the base exposure, the dermis demonstrated the strongest buffering capacity. At 30 min, intact skin demonstrated the strongest buffering capacity, while the dermis was ranked weakest (p < 0.01). Following washing, the pH values of the dermis more closely followed the baseline value; pH = 6.0 at 0 min and 10 min post-washing, compared to other layers. At 30 min post-washing, the intact skin’s pH values declined faster than the other layers (see Figure 1). The ability of HCl to modify skin pH was found to be comparable with the ability of NaOH, considering similar concentrations (see Figure 2).

Rate of Buffering Capacities

Buffering capacity: Figure 3 presents the rate of buffering capacity of skin layers which are calculated based on Eq. 1.

Zheng+Eq.+1

The dermis presented the greatest buffering capacity immediately after being exposed to acid or base. With time, the pH of the dermis changed less, compared with the other layers. The buffering capacity was highest in intact SC 30 min after application.

TEWL: The intact skin presented the lowest TEWL values while the dermis had the highest TEWL values— p = 0.041, compared with intact skin; the denuded SC model presented in the mid-values; p = 0.002 compared with the intact skin.

Discussion

The acidic property of normal skin plays a crucial role in regulating bacterial skin flora, sensitizing skin to cleansing procedures, and maintaining barrier homeostasis.1, 8, 9 Alterations in skin pH present a degree of diseased skin, undergoing acute or chronic modifications.9, 10 The baseline pH value for skin layers was within the 4 to 6 ± 0.2 range.1 Although human cadaver skin may maintain partial viability for several days,10 the alteration of human skin pH after death is not clear.

The correlation between human cadaver skin pH and in vivo measurements should be studied to provide better insights about the validity of in vitro skin pH investigations. Previously, skin pH and its buffering capacity were demonstrated to be measurable in vitro by applying 0.025, 0.05 and 0.1N of HCL and NaOH on human cadaver skin and detecting pH alterations post-dosing and -washing stages. This study demonstrated that pH values of different skin layers could be easily measured on human cadaver skin.

The results shown here reveal that the skin buffering capacity of different skin layers substantially differ from each other. This finding could be a step in understanding issues related to dermatopharmacology and dermatotoxicology. Further investigations may also clarify relative buffering capacity and percutaneous penetration. However, due to limitations of in vitro skin pH buffering capacity measurements, such as in vivo buffering mechanisms involving oxygen and carbon dioxide, and bioavailability considerations, the present technique cannot fully replace in vivo investigations. By investigating the correlations between the described study and in vivo measurements, the clinical implications of these investigations may be more fully understood.

The authors conceptualize the complexity of the interaction of base/acid on the skin layers to involve not only percutaneous penetration kinetic (PK) and aggression kinetic, but constant pH change—with measuring penetration. Insufficient data is provided to propose a PK/PH model but the present system could provide the basis for such. This information will be especially important for optimizing cosmetics and skin care formulations of relatively high and low pH levels. Furthermore, the industry awaits the development of reliable data of in vivo models.

Conclusion

This study investigated the in vitro buffering capacity of human skin layers after exposure with a topically applied model acid and base. NaOH and HCl solutions were applied to intact SC, denuded SC and dermis skin samples. Results indicated that immediately following acid or base exposure, the dermis had the highest buffering capacity while with time, the buffering capacity of intact SC predominates. These findings present the potential to better understand skin’s buffering capacity as it relates to dermatopharmacology and the dermatotoxicology of skin care foundations.

References

  1. J Levin and HI Maibach, Human skin buffering capacity: An overview, Skin Research and Technology 14(2) 121–126 (2008)
  2. H Zhai, HP Chan, S Farahmand and HI Maibach, Measuring human skin buffering capacity: An in vitro model, Skin Research and Technology 15(4) 470–475 (2009)
  3. V Kassis and J Sondergaard, Heat-separation of normal skin for epidermal and dermal prostaglandin analysis, Arch Dermatol Res 273(3–4) 301–306 (1982)
  4. J Welzel, pH and ions, in: E Berardesca, P Elsner, K-P Wilhelm and HI Maibach, eds, Bioengineering of the skin: Methods and instrumentation, CRC Press, Boca Raton, FL, USA (20050 pp 91–93
  5. C Ehlers, UI Ivens, ML M ller, T Senderovitz and J Serup, Comparison of two pH meters used for skin surface pH measurement: The pH meter ‘pH900’ from Courage & Khazaka versus the pH meter ‘1140’ from Mettler Toledo, Skin Res Technol 7 84–89 (2001)
  6. R Elkeeb, X Hui, H Chan, L Tian and HI Maibach, Correlation of transepidermal water loss with skin barrier properties in vitro: Comparison of three evaporimeters, Skin Res Technol 16(1) 9–15 (Feb 2010)
  7. HI Maibach and J Levin, Correlating transepidermal water loss and percutaneous absorption: An overview, in Skin Barrier: Chemistry of Skin Delivery Systems, JW Wiechers, ed, Allured Business Media, Carol Stream, IL, USA, ch 10 (2008) pp 91–96
  8. F Rippke, V Schreiner and HJ Schwanitz, The acidic milieu of the horny layer: New findings on the physiology and pathophysiology of skin pH, Am J Clin Dermatol 3(4) 261–72 (2002)
  9. K Chikakane and H Takahashi, Measurement of skin pH and its significance in cutaneous diseases, Clin Dermatol 13(4) 299–306 (1995)
  10. RC Wester, J Christoffel, T Hartway, N Poblete, HI Maibach and J Forsell, Human cadaver skin viability for in vitro percutaneous absorption: Storage and detrimental effects of heat-separation and freezing, Pharm Res 15(1) 82–84 (1998)
  11. Y Zheng, B Sotoodian, W Lai and HI Maibach, Buffering capacity of human skin layers: In vitro, Skin Research and Technology (2011)

This content is adapted from an article in GCI Magazine. The original version can be found here.

 

Close

Figure 1. Skin pH changes following NaOH exposure

Figure 1. Skin pH changes following NaOH exposure

Skin pH changes following NaOH exposure; note that the final measured pH did not reach baseline values, pH = 6.0; *P < 0.05, SC denuded model compared with intact SC; ▲ P < 0.05, dermis model compared with intact SC

Figure 2. Skin pH changes following HCl exposure

Figure 2. Skin pH changes following HCl exposure

Skin pH changes following HCl exposure; note that final measured pH values reached the baseline readings 30 min post-wash; *P < 0.05, SC denuded model compared with intact SC; ▲ P < 0.05, dermis model compared with intact SC.

Figure 3. Calculated buffering capacities

Figure 3. Calculated buffering capacities

Buffering capacities calculated based on Eq. 1, a) for NaOH and b) for HCl

Footnotes [Zheng 127(6)]

a The PM900 pH Meter and the TM 210 Tewameter used for this study are manufactured by Courage and Khazaka, Kolne, West Germany; and bAcaderm, Menlo Park, CA, USA.

Next image >