Figure 1. Ingredient list for Cellulite Suppress Formula

Ingredients: Water (aqua), Isododecane, Ethylhexyl Palmitate, Pentylene Glycol, Butylene Glycol, Glycerin, Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer, Caprylic/Capric Triglyceride, Gelidium Cartilagineum Extract (Rhodysterol, BiotechMarine), Laminaria Hyperborea Extract (Phycoboreane, BiotechMarine), Chenopodium Quinoa Seed Extract (Adipoless, Seppic), Sorbitan Laurate (and) Lauroyl Proline (Adiposlim, Seppic), Menthyl PCA, Centella Asiatica Extract, Aesculus Hippocastanum (Horse Chestnut) Seed Extract, Lecithin (vegetable), Menthol, Glycyrrhetinic Acid, Sodium Hyaluronate, Disodium EDTA, Phenoxyethanol, Caprylyl Glycol, Isoprene Glycol

Footnotes

^a Slim-Excess (INCI: Water (aqua) (and) Butylene Glycol (and) Sodium Chloride (and) Hydrolyzed Carrageenan (and) Xanthan Gum) is a product and trademark of BASF Beauty Care Solutions.

^bAdiposlim (INCI: Sorbitan Laurate (and) Lauroyl Proline) is a product and trademark of Seppic.

^cAdipoless (INCI: Chenopodium Quinoa Seed Extract) is a product and trademark of Seppic.

^dPhycoboreane (INCI: Laminaria Hyperborea Extract) is a product and trademark of BiotechMarine.

^eRhodysterol (INCI: Gelidium Cartilagineum Extract) is a product and trademark of BiotechMarine.

^f Cyberware Rapid 3D Digitizer is a product of Cyberware, Inc., Monterey, Calif., USA.

^g BTC-2000 is a product and trademark of SRLI Technologies, Skin Research Laboratory, Inc., Nashville, Tenn., USA.

Supporting Claims for Sulfo-carrabioses Solution

Here is how BASF tested the efficacy of its sulfo-carrabioses ingredient:⁵

Researchers evaluated the capacity of this active to trap spermine and spermidine in tubo. Spermine and spermidine are two positively charged molecules, so they bind with DNA, causing it to precipitate in solution. This is shown by an increase in the optical density (OD) in tubo, using spectrophotometry. A spermine and spermidine trap prevents the precipitation and causes a decrease in OD.

Researchers evaluated the effects of this active in inhibiting lipogenesis and inducing lipolysis in live adipocytes, compared to a negative control. Live adipocytes from human donors were obtained and grown in culture. To demonstrate lipogenesis, researchers used liquid scintillation to measure the incorporation of radioactive acetate into the adipocytes. To demonstrate lipolysis, an NEFA-C kit was used to assay the free fatty acids that were released by the adipocytes into the culture medium.

Researchers then evaluated pre-adipocyte proliferation in the presence of this active, compared to caffeine and negative control. Pre-adipocytes were obtained from human donors and grown in culture medium; after seven days of treatment, a Malassez cell was used to count the cells under optical microscope.

Researchers finally evaluated the slimming effect of this active, compared to a placebo. Instrumental data, clinical scores and self-evaluation questionnaires were obtained during and after an eight-week treatment period. Instrumental data included centimetric thigh circumference measurements and thigh volumes obtained using Fringe projection.

Supporting Claims for a Blend of Sorbitan Laurate and Lauroyl Proline

Here is how Seppic tested the efficacy of its blend of sorbitan laurate and lauroyl proline:⁸

Researchers evaluated the ingredient’s inhibition of the free fatty acid (FFA) content with adipocytes by showing that it inhibits the transformation of triglycerides into FFAs. The entry of non-metabolizable fluorescent fatty acid (5-methyl-BDY-dodecanoic acid) into mature adipocytes was measured with or without the ingredient and compared to both a positive (insulin) and negative control (cold).

Researchers evaluated the ingredient’s lipolytic activity. They showed that it causes the breakdown of triglycerides into FFAs, which is explained by an increase of cAMP, a key substance within the adipocytes for fat elimination. Researchers measured triglyceride lipolysis by assay of FFAs expelled from normal human adipocytes (obtained from male and female abdominal cosmetic surgery) with or without the ingredient and compared it to a positive control (caffeine).

Researchers evaluated the ingredient’s effect on cAMP levels in adipocytes. They measured the increase in cAMP levels in normal human adipocyes in the presence of the ingredient, compared to caffeine.

Finally, the ingredient’s ability to prevent the released FFAs from reforming into triglycerides was evaluated. By comparing the percentage increase in intracellular ATP relative to caffeine, the researchers demonstrated that, instead of reforming as triglycerides, the FFAs in the presence of the ingredient are recycled into energy as cellular ATP.

Table 1. Selected recent US Patents (P) and Patent applications (PA) for anticellulite ingredients