Here is how Seppic tested the efficacy of its blend of sorbitan laurate and lauroyl proline:8
Researchers evaluated the ingredient’s inhibition of the free fatty acid (FFA) content with adipocytes by showing that it inhibits the transformation of triglycerides into FFAs. The entry of non-metabolizable fluorescent fatty acid (5-methyl-BDY-dodecanoic acid) into mature adipocytes was measured with or without the ingredient and compared to both a positive (insulin) and negative control (cold).
Researchers evaluated the ingredient’s lipolytic activity. They showed that it causes the breakdown of triglycerides into FFAs, which is explained by an increase of cAMP, a key substance within the adipocytes for fat elimination. Researchers measured triglyceride lipolysis by assay of FFAs expelled from normal human adipocytes (obtained from male and female abdominal cosmetic surgery) with or without the ingredient and compared it to a positive control (caffeine).
Researchers evaluated the ingredient’s effect on cAMP levels in adipocytes. They measured the increase in cAMP levels in normal human adipocyes in the presence of the ingredient, compared to caffeine.
Finally, the ingredient’s ability to prevent the released FFAs from reforming into triglycerides was evaluated. By comparing the percentage increase in intracellular ATP relative to caffeine, the researchers demonstrated that, instead of reforming as triglycerides, the FFAs in the presence of the ingredient are recycled into energy as cellular ATP.