Figure 3. The penetration of Nile Red into pig skin

Following an 8-hr application period, the penetration of Nile Red into pig skin via: a) cream; b) SLN; c) NLC 1 (SLN + oleic acid); and d) NLC 2 (SLN + glyceryl tricaprylate/caprate); within the images, a = the stratum corneum, b = epidermis and c = dermis. Photos were produced by superimposing normal light and fluorescence images of the same area; black bar corresponds to 100 μm; reproduced with permission from Reference 15.