Figure 3. Epifluorescent image of corneocytes stained with Nile red as seen under the epifluorescence microscope

Fluorescence intensity was determined utilizing a circuit composed of a voltmeter integrated with various electronic modules. The digital readout was obtained using a computer program developed in-house that displays the count rate continuously. A typical epifluorescent image of corneocytes stained with the Nile red is shown here.