Figure 6. Live cell imaging using a bioorthogonal click approach

Figure 6. Live cell imaging using a bioorthogonal click approach; a) a normal live cell with no fluorescence; b) an azide-functionalized sugar is incorporated into the cellular membrane via an enzymatic pathway; c) a cyclooctyne functionalized with a fluorophore is clicked with the cell, which is now fluorescent and can be imaged; and d) bioorthogonal fluorescent labeling of live animals.