Figure 1. The tested complexes were designed to inhibit the NF-κB and JAK/STAT activated signaling pathways in keratinocytes to interrupt communication between the keratinocyte and immune cell, providing an immediate soothing effect.

The hypothesis was then to test, in human keratinocytes, the ability of the EG and PN complexes to inhibit the NF-κB and JAK/STAT signaling pathways and subsequent cytokine and chemokine expression and release leading to pro-inflammatory cross-talk and skin irritation (see Figure 1).