a William’s E medium was obtained from Lonza.
b Insulin was obtained from Sigma.
c Hydrocortisone also was obtained from Sigma.
d Primocin is a product of Invivogen, San Diego, CA, USA.
e OCT media was obtained from CellPath, UK.
f Alexa Fluor 488 is a product of InVitrogen.
g Fluoromount-G was obtained from Electron Microscopy Sciences.
h The Nikon Eclipse E600 microscope was used for this assessment.
j The Nikon DXM1200F camera with ACT-1C software was used for this assessment.
k The Vivacam camera is manufactured by Lucid, USA.
m Image Pro analysis software is manufactured by Media Cybernetics.